Gel extraction kit pdf

All pcr products were excised from the gel and purified using qiaquick gel extraction kit qiagen, germany. The gel diffusion buffer, which is not included in the kit, must be prepared prior to beginning the procedure. The genelute gel extraction kit is designed for isolating dna from agarose gels. Qiaex ii agarose gel extraction protocol this protocol is designed for the extraction of 40bp to 50kb dna fragments from 0. Qiaquick gel extraction kit protocol using a microcentrifuge. Zymoclean gel dna recovery kit supplied with capped columns d4007 d4008 50 200 zymoclean large fragment gel dna recovery kit supplied with uncapped columns d4045 d4046 25 100 zr96 zymoclean gel dna recovery kit supplied with 96well plates d4021 d4022 2 x 96 4 x 96 refer to page 2 for column design specifics in each kit. Dna purified by the sureextract pcrgel extraction kit is significantly concentrated than dna purified by other methods the unique buffer formulation of sureextract pcrgel. The qiaex ii and qiaquick gel extraction kit can be used to extract dna from polyacrylamide gels. Quick reference purelink quick gel extraction kit catalog numbers k210012 and k210025 publication part number 7015017 man0003719 revision date 7 march 2011 intended use. Qiaex ii gel extraction kit en print bookmark share pdf 48kb english format file size language download get adobe reader contact qiagen. The sureextract pcrgel extraction combo kit is designed for the direct purification of pcr products and for the purification of dna from taetbe agarose gels.

Qiaquick gel extraction kit the qiaquick gel extraction kit cat. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. Description this kit is designed for the purification of up to 10. Dna fragments of interest are extracted by solubilizing slices of an agarose gel. Qiaquick pcr purification, qiaquick nucleotide removal, and qiaquick gel extraction. Pcr cleanup, gel extraction machereynagel 01 2011, rev. Gel extraction kit and a comparable kit from company q following manufacturers recommended protocols.

Dna ladder was run on a 2% agarose gel and 4 different fragment sizes 200 bp, 500 bp, 1 kb, and 5 kb were recovered using omega bioteks e. Thermo scientific genejet gel extraction kit is designed for rapid and efficient purification of dna fragments from standard or lowmelting point agarose gels run in either tae or tbe buffer. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. Add 10l membrane binding solution per 10mg of gel slice. Spin columns supplied with the kit enable purification of up to 5 g of dna 70 bp10 kb in size, and the recovery of the purified dna comes up to 8595 %. This kit can also be used for dna cleanup from enzymatic reactions see page 8. Why is the concentration of dna very low after a gel extraction. Thermo scientific genejet gel extraction kit thermo fisher. Qiaquick gel extraction kit protocol using a microcentrifuge chen. Qiaex ii handbook qiaex ii agarose gel extraction protocol. Dna can be extracted and purified from agarose gels with different melting points in 30 minutes using purelink silica membranebased quick gel extrac. Electrophoretic analysis of recovered dna from an agarose gel compared to the control. The recovered dna is free from agarose and other impurities, and is compatible with restriction.

Transfer up to 300 mg of the gel slice into a microcentrifuge tube. Hiyield gelpcr dna fragments extraction kit 2 description the hiyield gel pcr dna fragments extraction kit is designed to recover or concentrate dna fragments 50bp 10kb from agarose gels, pcr or other enzymatic reactions. The size range for effective purification is about 70 bp 10 kb. Gel extraction kit spin protocol materials and equipment to be supplied by user. The zymoclean gel dna recovery kits are gel dna extraction kits that provide rapid purification of highquality dna from taetbebuffered agarose gels.

Lumipure dna gel extraction kit manual the kit allows rapid about 20 minutes and efficient od260od2801. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. For columns we recommend 4 c storage for periods greater than 1 year. The axyprep dna gel extraction kit employs optimized reagents in combination with a convenient miniprep column to purify dna fragments from either tae or tbe agarose gels regular and lowmelt. The kit utilizes a proprietary silicabased membrane technology in the form of a convenient spin column. Any precipitate that forms in the buffers during storage can be redissolved by incubating briefly at 37 c, then cooling to room temperature before use. Load more dna on the gel and take care that you excise the gel.

Genelute gel extraction procedure video the genelute gel extraction kit is designed for the rapid purification of 50 bp to 10 kb linear dna fragments and plasmids from standard or lowmelting agarose gels. Following electrophoresis, excise dna band from gel and place gel slice in a 1. Following page, oligonucleotides are first visualized by uv shadowing or ethidium bromide staining and then eluted in 14 hours using edtacontaining buffer. Add ethanol 96100% to buffer pe before use see bottle label for volume. The unique dual purpose application and high yield dna minicolumn make this kit exceptional value.

Purelink quick gel extraction kit catalog numbers k210012 and k210025 publication part number 7015017 man0003719 revision date 7 march 2011 intended use for research use only. Genelute gel extraction kit 70 purifications sigmaaldrich. For departmental related matters, please visit the department of. Dna fragments in a size range of 70 bp to 10 kb can be efficiently recovered. Polygel dna extraction kit combines the simplicity of page with the power of hibind technology to allow rapid and consistent recovery of ssdna or dsdna from acrylamide gels.

Qiaquick pcr purification kit protocols 18 using a microcentrifuge 18 using a vacuum manifold 19 qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. In the presence of a chaotropic salt, the agarose is dissolved and the dna binds to the fiber matrix. Add 1 gel volume isopropanol to the sample and mix. This protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or low melt agarose gels in tae or tbe.

Up to 400 mg agarose can be processed per spin column. Forceps are to be sterilized in fine science tools heat block at. This protocol is designed to extract and purify dna of 70 bp. This technology is based on binding dna to silicabased membrane, followed by subsequent wash steps and then elute pure readytouse dna. Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32. The purelink quick gel extraction kit allows you to rapidly and efficiently purify dna fragments from tae or tbe agarose gels of various percentages.

The thermo scientific genejet gel extraction kit should be stored at room temperature 1525 c. The gel solubilization solution dissolves an agarose gel slice from gels run in tbe or tae buffer. Not intended for any animal or human therapeutic or diagnostic use. The norgen dna gel extraction kit is designed for the rapid preparation and purification of dna frag ments that have been fractionated on agarose gels. Dnafragments yes yes yes yes oligonucleotides no yes no no ligation yes yes yes yes nicktranslation yes yes yes yes pcr yes. L of qg buffer were added to 1 volume 100 mg gel slice of gel containing excised dna fragment. The kit was tested in the extraction of dna fragments from an agarose gel according to the protocol described in the manual. Purelink quick gel extraction kit thermo fisher scientific. Monarch dna gel extraction kit reproducibly recovers dna over a broad range of molecular weights. Qiaquick pcr nucleotide qiaquick gel qiaquick gel purification kit removal kit extraction kit extraction kit alkalinephosphatase yes yes yes yes cdnasynthesis yes no no yes dnase, yes yes yes yes nucleasedigestion kinase. Qiagen qiaquick gel extraction kit 28704 and 28706. A mixture of 7 dna fragments ranging from 10 kb down to 0. Vortex and incubate at 5065c until gel slice is completely dissolved.

Empirical bioscience agarose gel extraction kit is designed to extract highyield dna from agarose gels with simultaneous removal of primer dimers, nucleotides, proteins, salt, agarose, ethidium bromide, and. Remove the extra agarose gel to minimize the size of the gel slice. The genelute gel extraction kit combines silicabinding technology with the convenience of a spin or vacuum column format. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of dna fragments from gels up to 400 mg slices or enzymatic reactions. The method employs a microspin cup that contains a silicabased fiber matrix. Banerjee lab ucla molecular, cell and developmental biology. For departmental related matters, please visit the department of molecular, cell, and developmental biology website.

The purified dna from a 50 l eluate was analyzed by electrophoresis on a 1% agarose gel. Wizardr sv gel and pcr cleanup system quick protocol. We use qiagen gel extraction kit and we have not faced such problem with concentration of dna. Heat block or water bath capable of 60c microcentrifuge capable of at least,000 x g vortexer nucleasefree 1. The minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube. Qiaex ii gel extraction kit en print bookmark share pdf 48kb english format file size language.